Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction.
(2007) Nano Letters : a journal dedicated to nanoscience and nanotechnology — Vol. 7, n° 3, p. 796-801 (2007)
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- Abstract
- The clinically important vancomycin antibiotic inhibits the growth of pathogens such as Staphylococcus aureus by blocking cell wall synthesis through specific recognition of nascent peptidoglycan terminating in D-Ala-D-Ala. Here, we demonstrate the ability of single-molecule atomic force microscopy with antibiotic-modified tips to measure the specific binding forces of vancomycin and to map individual ligands on living bacteria. The single-molecule approach presented here provides new opportunities for understanding the binding mechanisms of antibiotics and for exploring the architecture of bacterial cell walls.
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Gilbert, Y., Deghorain, M., Wang, L., Xu, B., Pollheimer, P. D., Gruber, H. J., Errington, J., Hallet, B., Haulot, X., Verbelen, C., Hols, P., & Dufrêne, Y. (2007). Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction. Nano Letters : a journal dedicated to nanoscience and nanotechnology, 7(3), 796-801. https://doi.org/10.1021/nl0700853 (Original work published 2007)