Unravelling the mechanisms by which prebiotics improve metabolic disorders : interactions between gut microbes and intestinal mucus
(2023)
Files
PhD_Thesis_Paone_Paola_Final.pdf
Restricted Access - Adobe PDF
- 40.72 MB
Details
- Authors
- Supervisors
- Cani, Patrice
- Abstract
- Obesity is associated with several metabolic alterations linked with specific changes of the gut microbiota and gut barrier disruption. The mucus layer is one of the major components of the gut barrier and it has been shown to be altered in mice fed high-fat diet (HFD), western-style diet or low-fiber diet, as well as in genetically obese mice and in type 2 diabetic patients. The aim of this thesis was to understand the mechanisms by which the gut microbiota might affect the mucus layer in the context of obesity and metabolic disorders. We used two prebiotic compounds: fructooligosaccharides (FOS) and 2’-fucosyllactose (2’FL), known to counteract the effects of HFD-induced obesity. We explored the expression of genes involved in gut barrier function, goblet cell differentiation, mucus production, secreted and transmembrane mucins, mucin glycosylation and secretion, and the gut microbiota composition. This thesis shows that FOS and 2’FL treatments prevent, at least in part, HFD-induced metabolic disorders by acting on the mucus layer. These data suggest that targeting the mucus and the gut microbiota could help to prevent obesity and related disorders.
- Affiliations
Citations
Paone, P. (2023). Unravelling the mechanisms by which prebiotics improve metabolic disorders : interactions between gut microbes and intestinal mucus. https://hdl.handle.net/2078.5/24779