Nanobiotechnology and Photodynamic therapy for ex vivo purging of malignant cells from ovarian cortex
(2023) International Conference of Nanoscience and Nanobiotechnology (ICONNANO 2023) — Location: Brasilia, Brazil (16.August.2023)
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- Abstract
- Autotransplantation of the cryopreserved OT is the most suitable option to preserve fertility for prepubertal girls and women that require immediate cancer treatment [1, 2]. Leukemia is the 12th most common cancer in Europe among prepubertal girls and women of reproductive age [4]. Unfortunately, once their health has been restored, autotransplantation of cryopreserved OT for leukemia patients is not advised due to the high risk of transferring malignant cells back to the patient, leading to leukemia recurrence [3]. To safely transplant the OT from these patients and restore their fertility, our goal here was to develop a PDT strategy to eliminate leukemia ex vivo. To this end, we designed OR141-loaded niosomes (ORN) to create the most effective formulation for ex vivo purging OT fragments from myelogenous leukemia cells. After establishing the best ORN formulation, our PDT approach was used to eradicate HL60 from ex vivo tumor models prepared by microinjection of cancer cell suspension in ovarian fragments [5]. Additionally, we evaluated the effect of ORN-based PDT on follicle density, survival, and tissue quality was evaluated. The ex vivo purging of tumor models demonstrated that the PDT strategy with ORN could selectively eradicate the malignant cells as evidenced by PCR and immunohistochemical analysis. Regarding the effect of our PDT approach on follicle population and OT quality, our results after xenotransplantation revealed no significant difference between the follicle density of control (non-treated, grafted OT) and ORN-treated groups (2.38 ± 0.63 and 3.21 ± 1.94 morphologically normal follicles/mm2, respectively). Our results showed that the purging procedure causes no significant impairing effect on follicles development and tissue quality, suggesting our novel PDT procedure could be a promising strategy to destroy leukemia cells in fragments of ovarian tissue allowing its safe transplantation in cancer survivors.
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Moghassemi, S., Dadashzadeh, A., Bentes Azevedo, R., & Andrade Amorim, C. (2023). Nanobiotechnology and Photodynamic therapy for ex vivo purging of malignant cells from ovarian cortex. International Conference of Nanoscience and Nanobiotechnology (ICONNANO 2023), Brasilia, Brazil. https://hdl.handle.net/2078.5/239134