Sequence analysis of three deficient mutants of cytochrome oxidase subunit I of Saccharomyces cerevisiae and their revertants.

Lemarre, P;Robineau, S;Colson, A M;Netter, P
(1994) Current Genetics : lower eukaryotes and organelles — Vol. 26, n° 5-6, p. 546-552 (1994)

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Authors
  • Lemarre, P
    Author
  • Robineau, S
    Author
  • Colson, A M
    Author
  • Netter, P
    Author
Abstract
Three respiratory-deficient mutants of cytochrome oxidase subunit I in the yeast mitochondrion have been sequenced. They are located in, or near, transmembrane segment VI, the catalytic core of the enzyme. Respiratory-competent revertants have been selected and studied. The mutant V244M was found to revert at the same site in valine (wild-type), isoleucine or threonine. The revertants of the mutant G251R were of three types: glycine (wild-type), serine and threonine at position 251. A search for second-site mutations was carried out but none were found. Among 60 revertants tested, the mutant K265M was found to revert only to the wild-type allele.
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Lemarre, P., Robineau, S., Colson, A. M., & Netter, P. (1994). Sequence analysis of three deficient mutants of cytochrome oxidase subunit I of Saccharomyces cerevisiae and their revertants. Current Genetics : lower eukaryotes and organelles, 26(5-6), 546-552. https://doi.org/10.1007/BF00309948 (Original work published 1994)