New insight on the molecular mechanisms conferring resistance to DMI fungicides in Fusarium culmorum

Hellin, Pierre;Legrève, Anne
(2016) The 31 st Meeting of the Fusarium working group of the Koninklijke Nederlandse Plantenziektekundige Vereniging — Location: Utrecht (26.October.2016)

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Abstract
Fusarium culmorum is an important mycotoxigenic plant pathogen causing diseases on a variety of crops and weeds. In Belgium and in the neighboring countries, this species has been shown to be the predominant species involved in Fusarium head blight on wheat in 2011. In an assay to characterise this species sensitivity towards triazoles, significant differences were found between strains from a mostly European collec­tion, with up to 10­fold differences in EC50. An F. culmorum strain resistant to triazoles was obtained by cultivating the sensitive UK99 strain in tebuconazole­amended media. The obtained resistant strain showed similar levels of fitness (e.g. growth and pathogenicity) than its parental strain and was more resistant to all members of the demethylation inhibitor (DMI) fungicides but not to other fungicide families tested. Taking advan­tage of the recently published genome of UK99, a RNA­Seq analysis was performed to compare the response of the resistant strain to that of its parental strain. Numerous genes were found to be differentially expressed between the two strains and some of them could be the key to decipher the triazole resistance mechanisms in F. culmorum. Among them, a transporter protein was overex­ pressed in both strains when subjected to tebuco­nazole but at a significantly higher level in the resistant strain than in its parental strain. The potential role of this transporter in triazole efflux was confirmed by comparing the expression of this gene in F. culmorum strains with distinct triazole sensitivity.
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Hellin, P., & Legrève, A. (2016). New insight on the molecular mechanisms conferring resistance to DMI fungicides in Fusarium culmorum. Gewasbescherming, 47(5), 135. (Original work published 2016)