Oral selenate improves glucose homeostasis and partly reverses abnormal expression of liver glycolytic and gluconeogenic enzymes in diabetic rats.

Becker, Dominique;Reul, Bénédicte;Tanju Ozçelikay, Arif;Buchet, Jean-Pierre;Brichard, Sonia;et.al.
(1996) Diabetologia : clinical and experimental diabetes and metabolism — Vol. 39, n° 1, p. 3-11 (1996)

Files

pdfdocument.pdf
  • Restricted Access
  • Adobe PDF
  • 1.05 MB
Request a copy

Details

Authors
  • Becker, DominiqueUCLouvain
    Author
  • Reul, BénédicteUCLouvain
    Author
  • Tanju Ozçelikay, ArifUCLouvain
    Author
  • Buchet, Jean-PierreUCLouvain
    Author
  • Henquin, Jean-ClaudeUCLouvain
    Author
  • Brichard, SoniaUCLouvain
    Author
Show more
Abstract
Selenium is a trace element that exerts certain insulin-like actions in vitro. In this study, we evaluated its in vivo effects on the glucose homeostasis of rats made diabetic and insulin-deficient by streptozotocin. Na2SeO4 was administered ad libitum in drinking water and/or food for 10 weeks. The elevated plasma glucose levels (approximately 25 mmol/l) and glucosuria (approximately 85 mmol/day) of untreated rats were decreased by 50 and 80%, respectively, by selenate treatment. The beneficial effect of selenate was also evident during oral and intravenous glucose tolerance tests: the integrated glucose responses were decreased by 40-50% as compared to those in untreated rats. These effects were not due to an increase in plasma insulin levels. Compared to non-diabetic rats, pancreatic insulin reserves were reduced by more than 90% in treated and untreated diabetic rats. The hepatic activities and mRNA levels of two key glycolytic enzymes, glucokinase and L-type pyruvate kinase were blunted in diabetic rats. They increased approximately two- to threefold after selenate treatment, to reach 40-75% of the values in non-diabetic rats. In contrast, elevated activity and mRNA levels of the gluconeogenic enzyme, phosphoenolpyruvate carboxykinase, were reduced by 40-65% after selenate administration. Since selenate induced a moderate decrease in body weight due to an anorexigenic effect, we checked that there was no improvement of glucose homeostasis or hepatic glucose metabolism in an additional group of calorie-restricted diabetic rats, which was weight-matched with the selenate group. In addition, no obvious toxic side-effects on the kidney or liver were observed in the rats receiving selenate. In conclusion, selenate induces a sustained improvement of glucose homeostasis in streptozotocin-diabetic rats by an insulin-like action, which involves partial correction of altered pretranslational regulatory mechanisms in liver metabolism.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Becker, D., Reul, B., Tanju Ozçelikay, A., Buchet, J.-P., Henquin, J.-C., & Brichard, S. (1996). Oral selenate improves glucose homeostasis and partly reverses abnormal expression of liver glycolytic and gluconeogenic enzymes in diabetic rats. Diabetologia : clinical and experimental diabetes and metabolism, 39(1), 3-11. https://doi.org/10.1007/BF00400407 (Original work published 1996)