Expression of prostate-specific membrane antigen in transitional cell carcinoma of the bladder: prognostic value?

Gala, Jean-Luc;Loric, S;Guiot, Yves;Denmeade, S R;Tombal, Bertrand;et.al.
(2000) Clinical cancer research : an official journal of the American Association for Cancer Research — Vol. 6, n° 10, p. 4049-4054 (2000)

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Authors
  • Gala, Jean-LucUCLouvain
    Author
  • Loric, S
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  • Guiot, YvesUCLouvain
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  • Denmeade, S R
    Author
  • Brasseur, FrancisUCLouvain
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  • De Nayer, PhilippeUCLouvain
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  • Van Cangh, PaulUCLouvain
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  • Tombal, Bertrandorcid-logoUCLouvain
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Abstract
The expression of Prostate-specific membrane antigen (PSMA) mRNA was assessed in the normal bladder urothelium (n = 9), transitional cell carcinoma (TCC) specimens (n = 52), TCC-derived cell lines (n = 3), and preoperative blood samples from TCC patients (n = 27). Specific PSMA mRNA was found in 100% of normal and malignant tissues and two cell lines. PSMA protein was detected in normal (n = 3) and malignant tissues (n = 4). Using a PSMA-specific substrate, PSMA enzymatic activity was found in two bladder cell lines and correlated with immunostaining. Seven of the 27 TCC preoperative blood samples were positive by reverse transcription-PCR. These preliminary results, obtained on a nonrandomized cohort of patients, correlated with tumor invasion (positive RT-PCR: 0% for pT < or = 2 versus 41% for pT > or = 3) and 2-year survival rate (81% in the PSMA-negative group versus 29% in the PSMA-positive group). Although the clinical usefulness of this assay requires confirmation in larger prospective randomized trials, current preliminary results suggest that a blood-borne PSMA mRNA PCR assay may be a useful tool to predict a poor outcome in TCC patients.
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Gala, J.-L., Loric, S., Guiot, Y., Denmeade, S. R., Gady, A., Brasseur, F., Heusterspreute, M., Eschwège, P., De Nayer, P., Van Cangh, P., & Tombal, B. (2000). Expression of prostate-specific membrane antigen in transitional cell carcinoma of the bladder: prognostic value? Clinical cancer research : an official journal of the American Association for Cancer Research, 6(10), 4049-4054. https://hdl.handle.net/2078.5/130996 (Original work published 2000)