Comparative evaluation of four phenotypic tests for the detection of carbapenemase-producing Gram-negative bacteria.
(2017) Journal of Clinical Microbiology — Vol. 55, n° 2, p. 510-518 (2017)
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- Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates i.e. the Neo-Rapid CARB kit (Rosco Diagnostica A/S), the RAPIDEC CARBA NP test (BioMérieux SA), the β CARBA test (Bio-Rad Laboratories N.V) and a home-made electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates (Enterobacteriaceae [n= 198], nonfermentative Gram-negative bacilli [n = 130]) with previously characterized resistance mechanisms to carbapenems.Among Enterobacteriaceae, BYG Carba test and β CARBA test showed excellent sensitivity (respectively 100% and 97,3%) and specificity (respectively 98,9% and 97,7%). The two other assays yielded poorer performances with sensitivity and specificity of 91,9% and 83,9% for RAPIDEC CARBA NP test and of 89,2% and 89,7% for Neo-Rapid CARB kit respectively. Among Pseudomonas spp., the sensitivity and specificity ranged respectively from 87.3% to 92.7% and from 88.2% to 94.1%. Finally, all tests performed poorly against Acinetobacter spp., with sensitivity and specificity respectively ranging from 27.3% to 75.8% and 75 to 100%. Among commercially available assays, the β CARBA test appeared the most convenient for routine use and showed the best overall performances, especially against OXA-48-like producers. The BYG Carba test confirmed its excellent performance against Enterobacteriaceae (100% sensitivity and 98.9% specificity).
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Noël, A., Huang, T.-D., Berhin, C., Hoebeke, M., Bouchahrouf, W., Yunus, S., Bogaerts, P., & Glupczynski, G. (2017). Comparative evaluation of four phenotypic tests for the detection of carbapenemase-producing Gram-negative bacteria. Journal of Clinical Microbiology, 55(2), 510-518. https://doi.org/10.1128/JCM.01853-16 (Original work published 2017)