Interleukin-9 upregulates mucus expression in the airways
(2000) American Journal of Respiratory Cell and Molecular Biology — Vol. 22, n° 6, p. 649-656 (2000)
Files
LOUAHED_AJRCMB2000.pdf
Restricted Access - Adobe PDF
- 532.48 KB
Details
- Authors
- Abstract
- Interleukin (IL)-9 has recently been shown to play an important role in allergic disease because its expression is strongly associated with the degree of airway responsiveness and the asthmatic-like phenotype, IL-9 is a pleiotropic cytokine that is active on many cell types involved in the allergic immune response, Mucus hypersecretion is a clinical feature of chronic airway diseases; however, the mechanisms underlying the induction of mucin are poorly understood. In this report, we show that IL-9 regulates the expression of a subset of mucin genes in lung cells both in vivo and in vitro. In vivo, the constitutive expression of IL-9 in transgenic mice results in elevated MUC2 and MUC5AC gene expression in airway epithelial cells and periodic acid-Schiff-positive staining (reflecting mucous glycogenates). Similar results were observed in C57BL/6J mice after IL-9 intratracheal instillation. In contrast, instillation of the T helper 1-associated cytokine interferon gamma failed to induce mucin production, In vitro, our studies showed that IL-9 also induces expression of MUC2 and MUC5AC in human primary lung cultures and in the human muccoepidermoid NCl-H292 cell line, indicating a direct effect of IL-9 on inducing mucin expression in these cells. Altogether, these results suggest that upregulation of mucin by IL-9 might contribute to the pathogenesis of human inflammatory airway disorders, such as asthma. These data extend the role of the biologic processes that IL-9 has on regulating the many clinical features of asthma and further supports the IL-9 pathway as a key mediator of the asthmatic response.
- Affiliations
Citations
Louahed, J., Toda, M., Jen, J., Hamid, Q., Renauld, J.-C., Levitt, R., & Nicolaides, N. (2000). Interleukin-9 upregulates mucus expression in the airways. American Journal of Respiratory Cell and Molecular Biology, 22(6), 649-656. https://doi.org/10.1165/ajrcmb.22.6.3927 (Original work published 2000)