Measurement of 1,25(OH)2D, the most biologically active form of vitamin D, circulating levels is relevant in several physiopathological states such as chronic kidney disease, parathyroid dysfunction, sarcoidosis, and vitamin D dependent rickets. Our study determined the performances of a novel automated 1,25(OH)2D immunoassay in a core-laboratory environment. We observed satisfactory analytical performances for this assay and an excellent agreement with a well established LC-MS/MS method. Furthermore, this assay allows a reduced TAT, integration in automated core-laboratories and potential consolidation with other tests of the bone and mineral metabolism.
Miller, N., & Gruson, D. (2016). Implementation of automated testing for 1,25-dihydroxyvitamin D: Return of experience from a core-laboratory. Clinical Biochemistry, 49(3), 298-301. https://doi.org/10.1016/j.clinbiochem.2015.10.017 (Original work published 2016)