Resynthesis of ATP in glycerinated fibres stretched during contraction
(1969) IIIrd intern Cong Biophysics, 271. — Location: Cambridge, Massachusets, USA (29.August.1969)
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- Bundles of glycerinated rabbit psoas fibres were made to contract in a Mg-ATP solution containing radioactive Pi. They were attached to strain gauges fastened on an ergometer. After 10 sec of isometric contraction, the fibres were slowly stretched during 3 sec and frozen at the end of the stretch. Specific activity of the ATP extracted from the stretched fibres was significantly (P = 0.02) higher than the one from the shortened fibres. The difference corresponded to a resynthesis of 2.2 pmoles ATP/ bundle, or to a gain of 22 ncal of free energy (taking 10 kcal/mole as ΔF for the synthesis of one ⁓P). The efficiency of the stretch amounted to 15 %. The results support the hypothesis that the energy coupling of the muscular contraction is reversible.
Citations
Gillis, J. M., & Maréchal, G. (1969). Resynthesis of ATP in glycerinated fibres stretched during contraction. Proc Cong Biophysics, 271. https://hdl.handle.net/2078.5/214731 (Original work published 1969)