The rise and future of CRISPR-based approaches for high-throughput genomics.
(2024) FEMS microbiology reviews — Vol. 48, n° 5 (2024)
Files
Vercauteren2024-FEMSreview.pdf
Open Access - Adobe PDF
- 2.62 MB
Details
- Authors
Fiesack, Simon 
Vonesch, Sibylle C
- Abstract
- Clustered regularly interspaced short palindromic repeats (CRISPR) has revolutionized the field of genome editing. To circumvent the permanent modifications made by traditional CRISPR techniques and facilitate the study of both essential and nonessential genes, CRISPR interference (CRISPRi) was developed. This gene-silencing technique employs a deactivated Cas effector protein and a guide RNA to block transcription initiation or elongation. Continuous improvements and a better understanding of the mechanism of CRISPRi have expanded its scope, facilitating genome-wide high-throughput screens to investigate the genetic basis of phenotypes. Additionally, emerging CRISPR-based alternatives have further expanded the possibilities for genetic screening. This review delves into the mechanism of CRISPRi, compares it with other high-throughput gene-perturbation techniques, and highlights its superior capacities for studying complex microbial traits. We also explore the evolution of CRISPRi, emphasizing enhancements that have increased its capabilities, including multiplexing, inducibility, titratability, predictable knockdown efficacy, and adaptability to nonmodel microorganisms. Beyond CRISPRi, we discuss CRISPR activation, RNA-targeting CRISPR systems, and single-nucleotide resolution perturbation techniques for their potential in genome-wide high-throughput screens in microorganisms. Collectively, this review gives a comprehensive overview of the general workflow of a genome-wide CRISPRi screen, with an extensive discussion of strengths and weaknesses, future directions, and potential alternatives.
- Affiliations
Citations
Vercauteren, S., Fiesack, S., Maroc, L., Verstraeten, N., Dewachter, L., Michiels, J., & Vonesch, S. C. (2024). The rise and future of CRISPR-based approaches for high-throughput genomics. FEMS microbiology reviews, 48(5). https://doi.org/10.1093/femsre/fuae020 (Original work published 2024)