Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from beta-lactam-resistant gram-negative bacteria.

Bogaerts, Pierre; Hujer, Andrea M; Naas, Thierry; de Castro, Roberta Rezende; Endimiani, Andrea; Nordmann, Patrice; Glupczynski, Gerald; Bonomo, Robert A

doi:10.1128/AAC.00353-11

Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from beta-lactam-resistant gram-negative bacteria.

Bogaerts, Pierre

;

Hujer, Andrea M

;

Naas, Thierry

;

de Castro, Roberta Rezende

;

Bonomo, Robert A

;et.al.

(2011) Antimicrobial Agents and Chemotherapy — Vol. 55, n° 9, p. 4457-4460 (2011)

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Authors

Bogaerts, PierreUCLouvain
Author
Hujer, Andrea M
Author
Naas, Thierry
Author
de Castro, Roberta Rezende
Author
Glupczynski, GeraldUCLouvain
Author
Bonomo, Robert A
Author

Abstract

A new commercial low-density microarray which identifies common extended-spectrum β-lactamase plasmid-mediated cephalosporinase genes, as well as carbapenemase (bla(KPC) and bla(NDM)) genes, was evaluated. We tested 207 clinical and reference/collection isolates of the Enterobacteriaceae possessing different bla genes. Overall, the sensitivity and specificity of the microarray were 100% for the detection of the plasmid-mediated bla(AmpC), bla(KPC), and bla(NDM) genes using bla gene sequencing as the reference method.

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Bogaerts, P., Hujer, A. M., Naas, T., de Castro, R. R., Endimiani, A., Nordmann, P., Glupczynski, G., & Bonomo, R. A. (2011). Multicenter evaluation of a new DNA microarray for rapid detection of clinically relevant bla genes from beta-lactam-resistant gram-negative bacteria. Antimicrobial Agents and Chemotherapy, 55(9), 4457-4460. https://doi.org/10.1128/AAC.00353-11 (Original work published 2011)