Protecting Effect of Cyclo-3-fort and its Constituents for Human Endothelial-cells Under Hypoxia
(1994) Clinical Hemorheology — Vol. 14, p. S15-S21 (1994)
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- ECV-304 endothelial cells derived from human umbilical cords were maintained for up to 48 hours under hypoxia in presence or absence of CYCLO 3 FORT and its individual constituents, namely Ruscus extract Hesperidine Methyl Chalcone and Ascorbic Acid. The cell survival was determined by the MTT tetrazolium test. Cell survival after 24 or 48 hours of hypoxia did not exceed 8 % while in presence of the drug preparation at 330 mu g/ml the cell survival was about 37%. and 25% respectively. The protection was dose dependent and observed even at 12 mu g/ml. Each of the constituents afforded a dose dependant protection against hypoxia at doses higher than 100 mu g/ml. There seems to be a synergistic effect among them, since the sum of the protecting effects conferred by each constituent individually is at least 5 times lower than that of CYCLO 3 FORT. On endothelial cells from porcine pulmonary arteries maintained in normoxia we have observed a dose dependant stimulation of mitochondrial succinate deshydrogenase activity by CYCLO 3 FORT, with an optimal effect at 250 mu g/ml. The stimulation of the oxidative metabolism which normally leads to the production of ATP has also been observed with the constituents of the drug preparation, mainly by Hesperidine Methyl Chalcone and Ascorbic Acid. Therefore the protection against hypoxia conferred to endothelial cells by CYCLO 3 FORT and its constituents could be due to the stimulation of the oxidative metabolism of those cells. These observations could contribute to the understanding of the therapeutic properties of CYCLO 3 FORT.
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Baurain, R., Dom, G., & Trouet, A. (1994). Protecting Effect of Cyclo-3-fort and its Constituents for Human Endothelial-cells Under Hypoxia. Clinical Hemorheology, 14, S15-S21. https://hdl.handle.net/2078.5/29280 (Original work published 1994)