Identification of mediators involved in the Follistatin actions on skeletal muscle : proteomic and transcriptomic approaches
(2017)
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- Authors
- Supervisors
- Thissen, Jean-Paul
- Abstract
- Follistatin has been shown to induce a marked increase in muscle mass. In addition, it impacts on muscle contractility and energy metabolism. However, the molecular mechanisms responsible for these phenotypic changes remain unclear. The aim of this thesis was to characterize the skeletal muscle proteome, transcriptome and secretome of mice overexpressing Follistatin in order to identify new mediators in the Follistatin actions on skeletal muscle. Our work provides molecular-level insights to explain the observed muscle phenotypic changes in both fast and slow muscles, including modifications in energy metabolism, fiber type, insulin and calcium signalings, as well as membrane repair and regeneration. Furthermore, we demonstrate that IGF-I and PAK1 are dispensable for the hypertrophic effect of Follistatin, in contrast to insulin. From a metabolic point of view, we show that Follistatin increases GLUT4 vesicles translocation independently of the PAK1 activity kinase. Finally, we provide the first putative secretome of skeletal muscle overexpressing Follistatin.
- Affiliations
Citations
Barbé, C. (2017). Identification of mediators involved in the Follistatin actions on skeletal muscle : proteomic and transcriptomic approaches. https://hdl.handle.net/2078.5/54038