Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum

Rakotoniriana, Erick Francisco S.;Rafamantanana, Mamy;Randriamampionona, Denis;Rabemanantsoa, Christian;Declerck, Stephan;et.al.
(2013) Antonie van Leeuwenhoek : international journal of general and molecular microbiology — Vol. 103, n° 1, p. 121-133 (2013)

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Authors
  • Rakotoniriana, Erick Francisco S.UCLouvain
    Author
  • Rafamantanana, MamyInstitut malgache de recherches appliquées, Antananarivo, Madagascar
    Author
  • Randriamampionona, DenisInstitut malgache de recherches appliquées, Antananarivo, Madagascar
    Author
  • Rabemanantsoa, ChristianInstitut malgache de recherches appliquées, Antananarivo, Madagascar
    Author
  • Munaut, FrançoiseUCLouvain
    Author
  • Corbisier, Anne-MarieUCLouvain
    Author
  • Quetin-Leclercq, JoëlleUCLouvain
    Author
  • Declerck, StephanUCLouvain
    Author
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Abstract
Thirty-one endophytic bacteria isolated from healthy leaves of Centella asiatica were screened in vitro for their ability to reduce the growth rate and disease incidence of Colletotrichum higginsianum, a causal agent of anthracnose. Isolates of Cohnella sp., Paenibacillus sp. and Pantoea sp. significantly stimulated the growth rate of C. higginsianum MUCL 44942, while isolates of Achromobacter sp., Acinetobacter sp., Microbacterium sp., Klebsiella sp. and Pseudomonas putida had no influence on this plant pathogen. By contrast, Bacillus subtilis BCA31 and Pseudomonas fluorescens BCA08 caused a marked inhibition of C. higginsianum MUCL 44942 growth by 46 and 82 %, respectively. Cell-free culture filtrates of B. subtilis BCA31 and P. fluorescens BCA08 were found to contain antifungal compounds against C. higginsianum MUCL 44942. Inoculation assays on in vitro-cultured plants of C. asiatica showed that foliar application of B. subtilis BCA31, three days before inoculation with C. higginsianum MUCL 44942, significantly reduced incidence and severity of the disease. The role of endophytic bacteria in maintaining the apparent inactivity of C. higginsianum MUCL 44942 in C. asiatica grown in the wild is discussed.
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Rakotoniriana, E. F. S., Rafamantanana, M., Randriamampionona, D., Rabemanantsoa, C., Urveg-Ratsimamanga, S., El Jaziri, M., Munaut, F., Corbisier, A.-M., Quetin-Leclercq, J., & Declerck, S. (2013). Study in vitro of the impact of endophytic bacteria isolated from Centella asiatica on the disease incidence caused by the hemibiotrophic fungus Colletotrichum higginsianum. Antonie van Leeuwenhoek : international journal of general and molecular microbiology, 103(1), 121-133. https://doi.org/10.1007/s10482-012-9791-2 (Original work published 2013)