Supernatant proteolytic activities of High-Five insect cells grown in serum-free culture
(2002) Biotechnology Letters — Vol. 24, n° 12, p. 965-969 (2002)
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- Authors
Agathos, Spiros N. 
- Abstract
- The proteolytic activity of High-Five insect cell culture supernatants was analysed using substrate gel electrophoresis (zymography). During growth in serum-free media, High-Five cells constitutively expressed and secreted proteases that were active on casein gel but not on gelatin or bovine serum albumin gels. Two main protease bands were visible at about 41-42 kDa and 32-33 kDa. By addition of various protease inhibitors in the incubation buffer, the proteases were identified as metalloproteases as complete and specific inhibition of the proteolytic activities was only obtained by 1,10-phenanthroline.
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Citations
Ikonomou, L., Peeters-Joris, C., Schneider, Y.-J., & Agathos, S. N. (2002). Supernatant proteolytic activities of High-Five insect cells grown in serum-free culture. Biotechnology Letters, 24(12), 965-969. https://doi.org/10.1023/A:1015692323167 (Original work published 2002)