Radiolabeling of Pluronic amphiphilic copolymer for adsorption studies.
(2003) Journal of Colloid and Interface Science — Vol. 259, n° 1, p. 163-170 (2003)
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- Abstract
- A new pathway for the radiolabeling of Pluronic PE6800 was developed. In a first step, the CH(2)-OH end groups of the copolymer were substituted by tosylates; in a second step these were reduced by [3H]-NaBH(4) to obtain tritiated chain ends. The final product was shown to be a mixture of native, tosylated, and reduced Pluronic containing 1 tritium atom per 1110 Pluronic molecules. The labeling procedure did not affect the molecular weight distribution nor the adsorption isotherm of the copolymer on polystyrene plates. A plateau value of about 0.7 microg/cm(2) is reached at a concentration in solution of 500 microg/ml, i.e., much lower than the cmc. Upon drying, the Pluronic adsorbed layer reorganizes in particles with a size of about 30 to 60 nm which cover about 15% of the substratum surface. This observation is of great importance for the design of protein-resistant surfaces by adsorption of Pluronic.
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De Cupere, V. M., Biltresse, S., Marchand-Brynaert, J., & Rouxhet, P. (2003). Radiolabeling of Pluronic amphiphilic copolymer for adsorption studies. Journal of Colloid and Interface Science, 259(1), 163-170. https://doi.org/10.1016/S0021-9797(02)00161-3 (Original work published 2003)