Partial within-animal calibration: A new calibration approach in lead optimisation high-throughput bioanalysis
(2007) Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences — Vol. 854, n° 1-2, p. 183-191 (2007)
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- Abstract
- The lead optimization phase of drug discovery requires high-throughput analyses for quantification in biological matrices and in plasma in particular. Over the last decade. some technical innovations allowed the pharmaceutical industry to improve the quality of the results. However, there is room for improvement. In this context, a new calibration strategy is proposed in this paper. Experiments were performed on dog plasma samples and it was showed that a within-animal calibration strategy can reduce the bias up to 20% and improve the precision up to 20%. However, a partial within-animal calibration is preferred to the full approach in order to avoid to many sample preparations. (C) 2007 Elsevier B.V. All rights reserved.
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Citations
Dewe, W., De Smet, M., Evrard, N., Culot, B., Lastelle, M., Saucez, G., Ingels, D., & Taillieu, X. (2007). Partial within-animal calibration: A new calibration approach in lead optimisation high-throughput bioanalysis. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 854(1-2), 183-191. https://doi.org/10.1016/j.jchromb.2007.04.018 (Original work published 2007)