Functional mapping reveals the importance of yeast cytochrome b C-terminal region in assembly and function of the bc(1) complex
(1997) FEBS Letters — Vol. 404, n° 1, p. 51-55 (1997)
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- Genetic and molecular analyses have been undertaken for four respiratory deficient mutants (mit(-)). The four mutations affect the C-terminal region of apocytochrome b. The frameshift (L263STOP) and non-sense (Q338STOP) mutations give rise to a truncated apocytochrome b. The mutant G337R conserves only 32% of its NADH oxidase activity which suggests that the presence of a positively charged amino acid in the transmembranous helix 7 of cytochrome b alters, either directly or indirectly, the bc(1) function, without affecting its assembly. The mutation G352V has a 65% loss of cytochrome b spectral content and prevents all of the mitochondrial respiratory activity. This leads us to believe that the glycine, conserved in position 352, may play a crucial role in bc(1) complex function. (C) 1997 Federation of European Biochemical Societies.
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Edderkaoui, B., Meunier, B., & ColsonCorbisier, A. (1997). Functional mapping reveals the importance of yeast cytochrome b C-terminal region in assembly and function of the bc(1) complex. FEBS Letters, 404(1), 51-55. https://doi.org/10.1016/S0014-5793(97)00085-9 (Original work published 1997)