Effects of storing pig ovaries at 4 or 20 degrees C for different periods of time on the morphology and viability of pre-antral follicles.

Lucci, C M;Schreier, L L;Machado, G M;Andrade Amorim, Christiani;Dobrinsky, J R;et.al.
(2007) Reproduction in Domestic Animals (Print): physiology, pathology, biotechnology Additional Title Information — Vol. 42, p. 76-82 (2007)

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Authors
  • Lucci, C MUniversidade de Brasília
    Author
  • Schreier, L LUniversidade de Brasília
    Author
  • Machado, G MUniversidade de Brasília
    Author
  • Andrade Amorim, Christianiorcid-logoUCLouvain
    Author
  • Dobrinsky, J RUS Department of Agriculture
    Author
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Abstract
The purpose of this study was to evaluate the effect of cooling ovarian tissue on pig pre-antral follicles. Ovaries were maintained in saline solution (0.9%) at 4 or 20 degrees C for 6, 12 or 18 h. After storage, pre-antral follicles were morphologically evaluated. While primordial follicles were not affected by the storage, the percentage of morphologically normal growing follicles was significantly reduced in ovarian tissue stored at 20 degrees C for 12 or 18 h. To test the viability of stored follicles, growing follicles isolated from ovaries stored at 4 degrees C for 18 h and at 20 degrees C for 6 h were cultured for 3 days. Follicles stored in either condition presented the same growth pattern in vitro as fresh follicles. We conclude that storage of pig ovaries at 4 degrees C for up to 18 h or at 20 degrees C for up to 6 h does not affect the morphology of growing follicles or their ability to grow in vitro.
Affiliations
  • Universidade de BrasíliaInstituto de Biologia

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Lucci, C. M., Schreier, L. L., Machado, G. M., Andrade Amorim, C., Báo, S. N., & Dobrinsky, J. R. (2007). Effects of storing pig ovaries at 4 or 20 degrees C for different periods of time on the morphology and viability of pre-antral follicles. Reproduction in Domestic Animals (Print): physiology, pathology, biotechnology Additional Title Information, 42, 76-82. https://doi.org/10.1111/j.1439-0531.2006.00734.x (Original work published 2007)