Nutrient Insulin Secretagogues Decrease Ca-45(2+) Efflux From Islet Cells By a Mechanism Other Than the Inhibition of the Na+-ca2+ Countertransport
(1985) FEBS Letters — Vol. 187, n° 1, p. 177-181 (1985)
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- <jats:p>The mechanism whereby nutrient insulin secretagogues decrease <jats:sup>45</jats:sup>Ca<jats:sup>2+</jats:sup> efflux from islet cells is controversial. It was studied with mouse islets perifused with Ca<jats:sup>2+</jats:sup>‐free solutions. In the presence of Na<jats:sup>+</jats:sup>, glucose and ketoisocaproate inhibited <jats:sup>45</jats:sup>Ca<jats:sup>2+</jats:sup> efflux by about 50%. Substitution of choline<jats:sup>+</jats:sup> salts for Na<jats:sup>+</jats:sup> salts decreased the efflux rate by 45%, but did not prevent glucose from decreasing it further. Ketoisocaproate also inhibited <jats:sup>45</jats:sup>Ca<jats:sup>2+</jats:sup> efflux, but less markedly than in an Na<jats:sup>+</jats:sup> medium. Omission of Na<jats:sup>+</jats:sup> decreased the efflux rate even when it was already lowered by glucose or ketoisocaproate. It is thus clear that nutrient insulin secretagogues decrease <jats:sup>45</jats:sup>Ca<jats:sup>2+</jats:sup> efflux from islet cells by a mechanism other than the inhibition of the Na<jats:sup>+</jats:sup>‐Ca<jats:sup>2+</jats:sup> countertransport, possibly by increasing sequestration of the ion in cellular organelles.</jats:p>
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Henquin, J.-C., Demiguel, R., Garrino, MG., Hermans, M., & Nenquin, M. (1985). Nutrient Insulin Secretagogues Decrease Ca-45(2+) Efflux From Islet Cells By a Mechanism Other Than the Inhibition of the Na+-ca2+ Countertransport. FEBS Letters, 187(1), 177-181. https://doi.org/10.1016/0014-5793(85)81237-0 (Original work published 1985)