Dominant TCR V alpha usage by virus and tumor-reactive T cells with wide affinity ranges for their specific antigens
(2002) European Journal of Immunology —
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- Abstract
- We have studied the TCR features and functional responses of three sets of human cytolytic T cell (CTL) clones, recognizing antigenic peptides presented by HLA-A2 and derived from the Epstein-Barr virus proteins BMLF1 and BRLF1 and from the melanoma protein Melan-A/MART-1. Within each set, a majority of clones used a recurrent Valpha region, even though they expressed highly diverse TCR beta chains and V(D)J junctional sequences. Functional assays and peptide/MHC multimer binding studies indicated that this restricted Va usage was not associated with the affinity/avidity of the CTL clones. The Valpha dominance, which may be a frequent feature of antigen-specific T cells, likely reflects a restricted geometry of TCR/peptide/MHC complexes, primarily determined by Valpha CDR.
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Trautmann, L., Labarrière, N., Jotereau, F., Karanikas, V., Gervois, N., Connerotte, T., Coulie, P., & Bonneville, M. (2002). Dominant TCR V alpha usage by virus and tumor-reactive T cells with wide affinity ranges for their specific antigens. European Journal of Immunology. https://doi.org/10.1002/1521-4141(200211)32:11<3181::AID-IMMU3181>3.0.CO;2-2