Plant-regeneration From Callus-culture of 3 Citrus Rootstocks
(1991) Plant Cell, Tissue and Organ Culture : an international journal on in vitro culture of higher plants — Vol. 24, n° 1, p. 29-34 (1991)
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- Abstract
- Complete plants were regenerated from callus derived embryo culture of Citrus aurantium, Poncirus trifoliata and of Carrizo citrange (C. sinensis x Poncirus trifoliata) via somatic embryogenesis and organogenesis. Isolated embryoids were differentiated on medium containing 1000 to 1500 mg l-1 malt extract. Both embryoids and shoots were obtained by the use of plant growth regulators: NAA and BAP. The highest frequency was obtained with 1 mg l-1 NAA + 5 mg l-1 BAP in Poncirus trifoliata and citrange but with 1 mg l-1 NAA + 10 mg l-1 BAP in C. aurantium. Rooting was promoted by a supplement of 1 mg l-1 GA3 or 1 mg l-1 NAA. GA3 enhanced stem elongation and rooting in embryoids and NAA stimulated adventitous root formation.
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Citations
Beloualy, N. (1991). Plant-regeneration From Callus-culture of 3 Citrus Rootstocks. Plant Cell, Tissue and Organ Culture : an international journal on in vitro culture of higher plants, 24(1), 29-34. https://doi.org/10.1007/BF00044262 (Original work published 1991)