CD32CD4 memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice.
(2021) iScience — Vol. 24, n° 1, p. 101881 [1-34] (2021)
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- CD32 has raised conflicting results as a putative marker of the HIV-1 reservoir. We measured CD32 expression in tissues from viremic and virally suppressed humanized mice treated relatively early or late after HIV-1 infection with combined antiretroviral therapy. CD32 was expressed in a small fraction of the memory CD4 T-cell subsets from different tissues in viremic and aviremic mice, regardless of treatment initiation time. CD32 memory CD4 T cells were enriched in cell-associated (CA) HIV-1 DNA but not in CA HIV-1 RNA as compared to the CD32CD4 fraction. Using multidimensional reduction analysis, several memory CD4CD32 T-cell clusters were identified expressing HLA-DR, TIGIT, or PD-1. Importantly, although tissue-resident CD32CD4 memory cells were enriched with translation-competent reservoirs, most of it was detected in memory CD32CD4 T cells. Our findings support that CD32 labels highly activated/exhausted memory CD4 T-cell subsets that contain only a small proportion of the translation-competent reservoir.
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Adams, P., Fievez, V., Schober, R., Amand, M., Iserentant, G., Rutsaert, S., Dessilly, G., Vanham, G., Hedin, F., Cosma, A., Moutschen, M., Vandekerckhove, L., & Seguin-Devaux, C. (2021). CD32CD4 memory T cells are enriched for total HIV-1 DNA in tissues from humanized mice. iScience, 24(1), 101881 [1-34]. https://doi.org/10.1016/j.isci.2020.101881 (Original work published 2021)