Light Induced Functionalization of PCL-PEG Block Copolymers for the Covalent Immobilization of Biomolecules.
(2009) Biomacromolecules — Vol. 10, n° 4, p. 966-974 (2009)
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- Abstract
- Functionalized poly-epsilon-caprolactone-block-polyethyleneglycol (PCL-PEG) amphiphilic copolymers were prepared to be constituents of nanocarriers used for the targeting of specific cells. Hence, we conceived a smooth and simple photografting methodology on these copolymers using a bifunctional molecular clip (O-succinimidyl-4-(p-azido-phenyl)butanoate). We prepared PCL-PEGs with pendent N-hydroxysuccinimide esters and studied the grafting with (3)H-lysine, which radioactivity was counted by LSC. Several parameters were investigated, such as behavior of homopolymers, initial concentrations, irradiation, and incubation durations. Evidences of a "PEG directed photografting" are discussed and this selectivity could be improved by a selective solvent technique. The photografting on different PCL-PEGs revealed a dependency of the rates to the crystallinity of the copolymers. Several controls by SEC, DLS, and TEM of the treated copolymers were realized. Lastly, the coupling of alpha-d-mannopyranoside ligand was performed, reaching amounts of 5400 nmol/g of PCL-PEG. This derivatized PCL-PEG enters in the preparation of nanocarriers used for the targeting of antigen presenting cells.
- Affiliations
Uliège CERM, B-4000 Liege CNRS, CERMAV F-38041 Grenoble, France
Citations
Pourcelle, V., Freichels, H., Stoffelbach, F., Auzély-Velty, R., Jérôme, C., & Marchand-Brynaert, J. (2009). Light Induced Functionalization of PCL-PEG Block Copolymers for the Covalent Immobilization of Biomolecules. Biomacromolecules, 10(4), 966-974. https://doi.org/10.1021/bm900027r (Original work published 2009)