Five challenges to bringing single-molecule force spectroscopy into living cells.
(2011) Nature Methods : techniques for life scientists and chemists — Vol. 8, n° 2, p. 123-127 (2011)
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- Authors
- Abstract
- In recent years, single-molecule force spectroscopy techniques have been used to study how inter- and intramolecular interactions control the assembly and functional state of biomolecular machinery in vitro. Here we discuss the problems and challenges that need to be addressed to bring these technologies into living cells and to learn how cellular machinery is controlled in vivo.
- Affiliations
Case Western Reserve University, Cleveland, Ohio, USA Department of Pharmacology Boston University, Boston Massachussetts, USA Medical Engineering and Physics Eidgenössische Technische Hochschule Zürich, Basel, Switzerland Department of Biosystems Science and Engineering Ludwig Maximillians University, Munich, Germany Applied Physics
Citations
Dufrêne, Y., Evans, E., Engel, A., Helenius, J., Gaub, H. E., & Müller, D. J. (2011). Five challenges to bringing single-molecule force spectroscopy into living cells. Nature Methods : techniques for life scientists and chemists, 8(2), 123-127. https://doi.org/10.1038/nmeth0211-123 (Original work published 2011)