Pomegranate and green tea extracts protect against ER stress induced by a high-fat diet in skeletal muscle of mice
(2014) European Journal of Nutrition — Vol. 54, n° 3, p. 377-389 (2014)
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- PURPOSE: We tested the hypothesis that polyphenol-rich extracts can reduce endoplasmic reticulum (ER) stress induced by a high-fat diet (HFD) in skeletal muscle of mice. METHODS: Mice were randomly assigned to four groups receiving during 20 weeks either a standard chow control (CTRL), or a HFD supplemented, or not, with pomegranate (HFD + P) or green tea (HFD + GT) extracts. After the nutritional intervention, mice were killed and gastrocnemius muscles were taken. Proteins and mRNA were measured by Western blot and RT-qPCR, respectively. RESULTS: Body weight gain and visceral fat were higher in HFD, HFD + P and HFD + GT than in CTRL. The markers of the unfolded protein response BiP, XBP1u, XBP1s and ATF4 were higher only in HFD. In HFD + P and HFD + GT, this increase was not observed except for CHOP, which was elevated in all HFD groups. HFD increased also markers of ubiquitin-proteasome pathway, autophagy and oxidative stress, which were kept low in HFD + P and HFD + GT groups. CONCLUSION: Our data provide evidence for a protective effect of pomegranate and green tea extracts against ER stress, oxidative stress and protein degradation induced by HFD in skeletal muscle. They give arguments for a usefulness of these natural nutritional compounds to fight against cellular dysfunctions related to fat excess.
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Rodriguez, J., Gilson, H., Jamart, C., Naslain, D., Pierre, N., Deldicque, L., & Francaux, M. (2014). Pomegranate and green tea extracts protect against ER stress induced by a high-fat diet in skeletal muscle of mice. European Journal of Nutrition, 54(3), 377-389. https://hdl.handle.net/2078.5/48663 (Original work published 2014)