Human but not mouse hepatocytes respond to interferon-lambda in vivo
(2014) PLoS One — Vol. 9, n° 1 (2014)
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- The type III interferon (IFN) receptor is preferentially expressed by epithelial cells. It is made of two subunits: IFNLR1, which is specific to IFN-lambda (IFN-γ) and IL10RB, which is shared by other cytokine receptors. Human hepatocytes express IFNLR1 and respond to IFN-γ. In contrast, the IFN-γ response of the mouse liver is very weak and IFNLR1 expression is hardly detectable in this organ. Here we investigated the IFN-γ response at the cellular level in the mouse liver and we tested whether human and mouse hepatocytes truly differ in responsiveness to IFN-γ. When monitoring expression of the IFN-responsive Mx genes by immunohistofluorescence, we observed that the IFN-γ response in mouse livers was restricted to cholangiocytes, which form the bile ducts, and that mouse hepatocytes were indeed not responsive to IFN-γ. The lack of mouse hepatocyte response to IFN-γ was observed in different experimental settings, including the infection with a hepatotropic strain of influenza A virus which triggered a strong local production of IFN-γ. With the help of chimeric mice containing transplanted human hepatocytes, we show that hepatocytes of human origin readily responded to IFN-γ in a murine environment. Thus, our data suggest that human but not mouse hepatocytes are responsive to IFN-γ in vivo. The non-responsiveness is an intrinsic property of mouse hepatocytes and is not due to the mouse liver micro-environment. © 2014 Hermant et al.
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Hermant, P., Demarez, C., Mahlakõiv, T., Staeheli, P., Meuleman, P., & Michiels, T. (2014). Human but not mouse hepatocytes respond to interferon-lambda in vivo. PLoS One, 9(1). https://doi.org/10.1371/journal.pone.0087906 (Original work published 2014)