Saccharomyces boulardii produces in rat small intestine a novel protein phosphatase that inhibits Escherichia coli endotoxin by dephosphorylation.

Buts, Jean-Paul;Dekeyser, Nadine;Stilmant, Catherine;Delem, Emilie;Sokal, Etienne;et.al.
(2006) Pediatric Research : international journal of human developmental biology — Vol. 60, n° 1, p. 24-29 (2006)

Files

21528.pdf
  • Restricted Access
  • Adobe PDF
  • 3.52 MB
Request a copy

Details

Authors
  • Buts, Jean-PaulUCLouvain
    Author
  • Dekeyser, NadineUCLouvain
    Author
  • Stilmant, CatherineUCLouvain
    Author
  • Delem, Emilie
    Author
  • Smets, FrançoiseUCLouvain
    Author
  • Sokal, EtienneUCLouvain
    Author
Show more
Abstract
Using a polyclonal antibody raised against a highly conserved sequence of 38 amino acids containing the activation site (VTDSAAGAT) common to mammalian and yeast alkaline phosphatases (AP), we identified in decapsidated Saccharomyces boulardii a protein phosphatase detected by autoradiography as a single signal (63 kD). Using an affinity chromatography column, the protein phosphatase could be concentrated 39.1-fold and presented as a doublet of two subunits. Compared with rat and bovine purified intestinal AP, the enzyme from S. boulardii had a greater ability to dephosphorylate the lipopolysaccharide (LPS) of Escherichia coli 055B5. When tested in vivo, intraperitoneal injection of intact LPS to rats produced, after 9 h, 100 ng/mL of circulating tumor necrosis factor-alpha with inflammatory lesions and apoptotic bodies in the liver and the heart, whereas rats injected with partially dephosphorylated LPS produced only 40 ng/mL tumor necrosis factor-alpha without organic lesions. In conclusion, S. boulardii is able to inhibit toxicity of E. coli surface endotoxins by the release of a protein phosphatase exhibiting a great capacity of dephosphorylation.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Buts, J.-P., Dekeyser, N., Stilmant, C., Delem, E., Smets, F., & Sokal, E. (2006). Saccharomyces boulardii produces in rat small intestine a novel protein phosphatase that inhibits Escherichia coli endotoxin by dephosphorylation. Pediatric Research : international journal of human developmental biology, 60(1), 24-29. https://doi.org/10.1203/01.pdr.0000220322.31940.29 (Original work published 2006)