Enhancing dendritic cell-based vaccination through in situ photodynamic therapy-induced ferroptosis : insights from 3D immunospheroid to mouse peritoneal carcinomatosis models
(2025)
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Details
- Authors
- Supervisors
- Feron, Olivier
- Abstract
- Peritoneal carcinomatosis (PC) is an aggressive, immune-resistant cancer with limited options. Here, we show that photodynamic therapy (PDT) remodels the tumor microenvironment to boost dendritic cell (DC)-based immunotherapy. Using 3D tumor spheroids and an orthotopic PC model, we find that PDT triggers ferroptosis-dependent immunogenic cell death (ICD), notably via the non-enzymatic lipid peroxidation pathway, enhancing DC infiltration and T cell activation. We implement a minimally invasive laparoscopic PDT using the LED light of a conventional endoscope, enabling in situ tumor targeting and fully personalized immune activation. Combined with intraperitoneal injection of immature DCs, this approach elicits robust T cell memory responses tailored to the patient’s tumor antigens. Our findings establish PDT as a clinically relevant ICD inducer that converts immune-excluded tumors into immunogenic niches and supports its use in next-generation, patient-specific DC vaccines.
- Affiliations
Citations
Degavre, C. (2025). Enhancing dendritic cell-based vaccination through in situ photodynamic therapy-induced ferroptosis : insights from 3D immunospheroid to mouse peritoneal carcinomatosis models. https://hdl.handle.net/2078.5/246632