Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping.

Delmée, Michel;Depitre, C.;Corthier, G.;Ahoyo, A;Avesani, V.
(1993) Journal of Clinical Microbiology — Vol. 31, n° 9, p. 2526-2528 (1993)

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Authors
  • Delmée, MichelUCLouvain
    Author
  • Depitre, C.
    Author
  • Corthier, G.
    Author
  • Ahoyo, A
    Author
  • Avesani, V.
    Author
Abstract
An enzyme-linked immunoassay (ELISA) with 11 Clostridium difficile serogroup-specific antisera was applied for serogrouping of C. difficile colonies from 314 consecutive positive fecal samples. Two hundred forty-nine strains (79%) were correctly serogrouped, 57 (18%) belonged to serogroups other than the 11 which were evaluated and gave a negative reaction with all antisera, and 8 isolates (2.5%) did not react with their corresponding antisera. ELISA is a rapid and reliable method for serogrouping C. difficile and should allow for the automation of this procedure.
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Delmée, M., Depitre, C., Corthier, G., Ahoyo, A., & Avesani, V. (1993). Use of an enzyme-linked immunoassay for Clostridium difficile serogrouping. Journal of Clinical Microbiology, 31(9), 2526-2528. https://hdl.handle.net/2078.5/74548 (Original work published 1993)