Analysing ambiguities in trypanosomatids taxonomy by barcoding.
(2020) Memórias do Instituto Oswaldo Cruz — Vol. 115, p. e200504 [1-14] (2020)
Files
2020_Boucinha_Mem_Inst_Oswaldo_Cruz.pdf
Open Access - Adobe PDF
- 677.81 KB
Details
- Authors
d'Avila-Levy, Claudia Masini 
- Abstract
- BACKGROUND: Biodiversity screens and phylogenetic studies are dependent on reliable DNA sequences in public databases. Biological collections possess vouchered specimens with a traceable history. Therefore, DNA sequencing of samples available at institutional collections can greatly contribute to taxonomy, and studies on evolution and biodiversity. METHODS: We sequenced part of the glycosomal glyceraldehyde phosphate dehydrogenase (gGAPDH) and the SSU rRNA (V7/V8) genes from 102 trypanosomatid cultures, which are available on request at www.colprot.fiocruz.br. OBJECTIVE: The main objective of this work was to use phylogenetic inferences, using the obtained DNA sequences and those from representatives of all Trypanosomatidae genera, to generate phylogenetic trees that can simplify new isolates screenings. FINDINGS: A DNA sequence is provided for the first time for several isolates, the phylogenetic analysis allowed the classification or reclassification of several specimens, identification of candidates for new genera and species, as well as the taxonomic validation of several deposits. MAIN CONCLUSIONS: This survey aimed at presenting a list of validated species and their associated DNA sequences combined with a short historical overview of each isolate, which can support taxonomic and biodiversity research and promote culture collections.
- Affiliations
Citations
Boucinha, C., Caetano, A. R., Santos, H. L., Helaers, R., Vikkula, M., Branquinha, M. H., Dos Santos, A. L. S., Grellier, P., Morelli, K. A., & d’Avila-Levy, C. M. (2020). Analysing ambiguities in trypanosomatids taxonomy by barcoding. Memórias do Instituto Oswaldo Cruz, 115, e200504 [1-14]. https://doi.org/10.1590/0074-02760200504 (Original work published 2020)