Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography

Kpoviessi, Dossou Sika Salomé;Gbaguidi, Fernand;Gbénou, Joachim;Accrombessi, Georges;Quetin-Leclercq, Joëlle;et.al.
(2008) Journal of Pharmaceutical and Biomedical Analysis — Vol. 48, n° 4, p. 1127-1135 (2008)

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Authors
  • Kpoviessi, Dossou Sika SaloméUCLouvain
    Author
  • Gbaguidi, Fernand
    Author
  • Gbénou, Joachim
    Author
  • Accrombessi, Georges
    Author
  • Quetin-Leclercq, JoëlleUCLouvain
    Author
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Abstract
An accurate and sensitive method, combining soxhlet extraction, solid phase-extraction and capillary gas chromatography is described for the quantitative determination of one triterpene (lupeol) and three sterols (stigmasterol, campesterol and beta-sitosterol) and the detection of another triterpene (alpha-amyrin) from the aerial part of Justicia anselliana. This is the first method allowing the quantification of sterols and triterpenes in this plant. It has been fully validated in order to be able to compare the sterol and triterpene composition of different samples of J. anselliana and therefore help to explain the allelopathic activity due to these compounds. This method showed that the aerial part of J. anselliana contained (292+/-2)mg/kg of lupeol, (206+/-1)mg/kg of stigmasterol, (266+/-2)mg/kg of campesterol and (184+/-9)mg/kg of beta-sitosterol.
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Kpoviessi, D. S. S., Gbaguidi, F., Gbénou, J., Accrombessi, G., Moudachirou, M., Rozet, E., Hubert, P., & Quetin-Leclercq, J. (2008). Validation of a method for the determination of sterols and triterpenes in the aerial part of Justicia anselliana (Nees) T. Anders by capillary gas chromatography. Journal of Pharmaceutical and Biomedical Analysis, 48(4), 1127-1135. https://doi.org/10.1016/j.jpba.2008.08.036 (Original work published 2008)