Force probing surfaces of living cells to molecular resolution

Müller, Daniel J.;Helenius, Jonne;Alsteens, David;Dufrêne, Yves
(2009) Nature Chemical Biology — Vol. 5, n° 6, p. 383-390 (2009)

Files

999-Dufrene-Muller-2009.pdf
  • Restricted Access
  • Adobe PDF
  • 1.05 MB
Request a copy

Details

Authors
  • Müller, Daniel J.Biotechnology Center, Technische Universität Dresden, Dresden, Germany.
    Author
  • Helenius, JonneBiotechnology Center, Technische Universität Dresden, Dresden, Germany.
    Author
  • Alsteens, Davidorcid-logoUCLouvain
    Author
  • Dufrêne, YvesUCLouvain
    Author
Abstract
Biological processes rely on molecular interactions that can be directly measured using force spectroscopy techniques. Here we review how atomic force microscopy can be applied to force probe surfaces of living cells to single-molecule resolution. Such probing of individual interactions can be used to map cell surface receptors, and to assay the receptors' functional states, binding kinetics and landscapes. This information provides unique insight into how cells structurally and functionally modulate the molecules of their surfaces to interact with the cellular environment.
Affiliations

Citations

  • APA
  • Chicago
  • FWB

Müller, D. J., Helenius, J., Alsteens, D., & Dufrêne, Y. (2009). Force probing surfaces of living cells to molecular resolution. Nature Chemical Biology, 5(6), 383-390. https://doi.org/10.1038/nchembio.181 (Original work published 2009)