1',5'-anhydrohexitol oligonucleotides: Hybridisation and strand displacement with oligoribonucleotides, interaction with RNase H and HIV reverse transcriptase
(1997) Chemistry: A European Journal — Vol. 3, n° 9, p. 1513-1520 (1997)
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- Abstract
- Hexitol nucleic acids (HNAs) with four natural bases form stable and sequence-selective duplexes with RNA. This was investigated by T-m determinations and gel shift experiments. The CD spectra of an HNA-RNA duplex show similarities with the CD spectra of the A-form of dsRNA. Single-stranded HNAs are able to induce strand displacement in a double-stranded RNA sequence. An HNA-RNA duplex is a poor substrate for RNase H, and can inhibit the RNase H-mediated cleavage of a natural DNA-RNA substrate. The HNA-RNA hybrid enhances the activity of HIV reverse transcriptase.
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Hendrix, C., Rosemeyer, H., DeBouvere, B., Vanaerschot, A., Seela, F., & Herdewijn, P. (1997). 1′,5′-anhydrohexitol oligonucleotides: Hybridisation and strand displacement with oligoribonucleotides, interaction with RNase H and HIV reverse transcriptase. Chemistry: A European Journal, 3(9), 1513-1520. https://doi.org/10.1002/chem.19970030920 (Original work published 1997)