THE BEST SOURCE OF ISOLATED STROMAL CELLS FOR THE ARTIFICIAL OVARY: MEDULLA OR CORTEX, CRYOPRESERVED OR FRESH?
(2015)
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- Background: To restore fertility in patients at risk of ovarian metastases, we aim to develop an artificial ovary by grafting isolated frozen-thawed ovarian follicles and ovarian cells (OCs) embedded in a matrix. Objective: To determine the best source of OCs for the artificial ovary: medulla or cortex, cryopreserved or fresh. Material and Methods: Ovarian tissue was retrieved from 7 patients with one half processed as fresh (fresh group) and the other half cryopreserved and thawed before processing (frozen group). In each group, OCs were isolated separately from the cortex and medulla, and their viability was tested. Fifty thousand OCs were then encapsulated in fibrin and grafted to peritoneal pockets in nude mice. Grafts recovered after 7 days were analyzed by immunohistochemistry for OC survival (vimentin), proliferation (Ki67), vascularization (double CD34) and cell apoptosis (TUNEL). Results: Cryopreservation decreased OC yield (-2804 cells/mg, p=0.015) and viability (-9.72%, p=0.052) before grafting and had an important (5-fold, p=0.2) but non-significant negative impact on OC survival in grafts. The medulla yielded many more cells(+3841 cells/mg, p0.001) with higher viability (+18.23%, p0.001) than did the cortex. Moreover, grafts with cells from the medulla exhibited a statistically significant 6.44- and 2.47-fold increase in human and total vascular surface area respectively. Conclusion: Fresh medullary tissue therefore appears to be the best source of OCs for the artificial ovary thanks to an increased isolated cell yield, better cell viability after isolation and improved graft vascularization
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Soares M, & et al. (2015). THE BEST SOURCE OF ISOLATED STROMAL CELLS FOR THE ARTIFICIAL OVARY: MEDULLA OR CORTEX, CRYOPRESERVED OR FRESH? https://hdl.handle.net/2078.5/89399